amy ikui

Ikui, Amy

Academic Appointments:

Professor, Biology Department, Brooklyn College, The City University of New York
Faculty, Biology and Biochemistry PhD Programs, The Graduate Center, The City University of New York

Degree(s)

Ph.D. (Pharmacology) Albert Einstein College of Medicine 2003

Research Focus:

Amy Ikui’s laboratory studies DNA replication and cell division using single cell organisms, Saccharomyces cerevisiae (budding yeast) and Chlamydomonas reinhardtii (green algae), with the goal of understanding the molecular mechanism of cell growth control in eukaryotes.

  1. We aim to understand how yeast cell senses its cell size through a proper cell growth and cell division control. This study focuses on identifying cell size surveillance systems to maintain cell size using S. cerevisiae as a model system.
  2. Cdc6 is a eukaryotic DNA replication protein which plays a role in cell proliferation. Our goal is to understand how Cdc6 protein level is regulated by kinase and phosphatase in cerevisiae.
  3. We use Chlamydomonas reinhardtii to study a mechanism of DNA replication in plant kingdom. This project focuses on pre-replicative complex which triggers DNA replication. Our goal is to understand how DNA replication is controlled in plants.

 

Selected Publications

Schroeder L and Ikui AE. Tryptophan confers resistance to SDS-associated cell membrane stress in Saccharomyces cerevisiae PLOS One (2019) 14 (3): e0199484 doi: 10.1371/journal.pone.0199484

Kono K, Ikui AE. A new cell cycle checkpoint senses plasma membrane/cell wall damage in budding yeast BioEssays. (2017) 39(4) doi: 10.1002/bies.201600210

Kono K, Al-Zain A, Schroeder L, Nakanishi M, Ikui AE. Plasma membrane/cell wall perturbation activates a novel cell cycle checkpoint in S. cerevisiae. PNAS (2016) 113(25) 6910-15.

Al-Zain A, Schroeder L, Sheglov A, Ikui AE. Cdc6 degradation requires phospho-degron created by GSK-3 and Cdk1 for SCFCdc4 recognition in S. cerevisiae. MBoC. (2015) 26(14):2609-19. 

Ikui AE, Rossio V, Schroeder L, Yoshida S. A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication. PLOS Genetics. (2012) 8(12) e1003099.

Grants over the last 5 years

(Active)

NIH SC1 GM121242 Ikui (PI) 4/1/2017-3/31/2021
Cell cycle checkpoint induced by plasma membrane damage in S. cerevisiae
$1,426,600 total cost